Proton translocation coupled to methanogenesis from methanol + hydrogen inMethanosarcina barkeri
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چکیده
منابع مشابه
Bioenergetics of methanogenesis from acetate by Methanosarcina barkeri.
Methane formation from acetate by resting cells of Methanosarcina barkeri was accompanied by an increase in the intracellular ATP content from 0.9 to 4.0 nmol/mg of protein. Correspondingly, the proton motive force increased to a steady-state level of -120 mV. The transmembrane pH gradient however, was reversed under these conditions and amounted to +20 mV. The addition of the protonophore 3,5,...
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The suitability of ubiquinol(1) and duroquinol as pulse reductants for initiating respirationdriven proton translocation by aerobic ox heart mitochondria was investigated. At 25 degrees C the V(max.) for oxidation was close to 280nmol of quinol oxidized/min per mg of protein, and the K(m) values were 8mum for ubiquinol(1) and 28mum for duroquinol. Pulses of ubiquinol(1) and duroquinol were rapi...
متن کاملUncoupling of Methanogenesis from Growth of Methanosarcina barkeri by Phosphate Limitation.
Production of methane by Methanosarcina barkeri from H(2)-CO(2) was studied in fed-batch culture under phosphate-limiting conditions. A transition in the kinetics of methanogenesis from an exponentially increasing rate to a constant rate was due to depletion of phosphate from the medium. The period of exponentially increasing rate of methanogenesis was extended by increasing the initial concent...
متن کاملA simple energy-conserving system: proton reduction coupled to proton translocation.
Oxidative phosphorylation involves the coupling of ATP synthesis to the proton-motive force that is generated typically by a series of membrane-bound electron transfer complexes, which ultimately reduce an exogenous terminal electron acceptor. This is not the case with Pyrococcus furiosus, an archaeon that grows optimally near 100 degrees C. It has an anaerobic respiratory system that consists ...
متن کاملMethanogenesis from Choline by a Coculture of Desulfovibrio sp. and Methanosarcina barkeri.
A sulfate-reducing vibrio was isolated from a methanogenic enrichment with choline as the sole added organic substrate. This organism was identified as a member of the genus Desulfovibrio and was designated Desulfovibrio strain G1. In a defined medium devoid of sulfate, a pure culture of Desulfovibrio strain G1 fermented choline to trimethylamine, acetate, and ethanol. In the presence of sulfat...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1987
ISSN: 0014-5793
DOI: 10.1016/0014-5793(87)80112-6